Figure 1. Synchrony.

A. Samples from elutriation A were double stained with calcofluor (for septa) and DAPI (for nuclei). Cells were assayed for initiation of anaphase by scoring cells with two nuclei but no septum (binucleates, open circles). The cells were also scored for septation (filled squares).

B. Cells from elutriation B were assayed for septation by phase contrast microscopy.

C. Cells from the cdc25-22 block release were assayed for septation by phase contrast microscopy.