Figure 7. Cluster Analysis of the top 750 cell cycle regulated genes.

Gene expression data from all experiments were clustered by a hierarchical method (Eisen et al.). Every row is a gene; every column is an array. Red signifies up-regulation (i.e., an experiment/control ratio greater than 1); green signifies down-regulation (i.e., an experiment/control ratio less than 1). Black is a ratio close to 1, and grey is missing data. Dynamic range is 16-fold from reddest red to greenest green. The time in hours since the beginning of timecouse experiments is shown in black numerals at the top of the Figure. Peaks in septation index are marked with purple rectangles at the top and bottom of the Figure. Clusters discussed in the text are marked with blocks of color. Data for the cdc10-C4 (asynchronous cells with the hyperactive allele cdc10-C4), ace2 OE (asynchronous cells over-expressing ace2), ace2 (asynchronous ace2 cells), sep1 OE (asynchronous cells over-expressing sep1), and sep1 (asynchronous sep1 cells) are taken from Rustici et al. cdc10 encodes a component of the MBF transcription factor; ace2 encodes the Ace2 transcription factor, and sep1 encodes a forkhead transcription factor. Other experiments are described in Materials and Methods.